self promotion

October 28, 2009 at 1:29 pm | | cool results, literature

But what is a blog for anyway? Advertising! Here’s my most recent paper:


“Azido Push−Pull Fluorogens Photoactivate to Produce Bright Fluorescent Labels” J. Phys. Chem. B

Sorry for the lame post, but I’ve been too busy to write cool things…


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  1. congrats! classy use of the CFL lightbulb, i like it.

    fairly certain this is a naive question, but is there an accepted quantum yield in your field to describe fluorophores as bright? how does the quantum yield of your amino fluorogens compare to other single molecule labels?

    Comment by joel — October 28, 2009 #

  2. the quantum yield of the dark form is negligible … unmeasurable.

    the “bright” forms are at least measurable. i’d say that 20% is bright. 95% is very bright. no accepted standard, though. Cy3 is like 15%, while some rhodamines are 95%.

    the activated dyes i report with 1% QY is in ethanol, but they are much brighter in viscous or rigid environments (e.g. 60-98%). for instance, in the cell membrane, they are very bright and we can easily see single molecules of them.

    Comment by sam — October 28, 2009 #

  3. Surely it’s incorrect to be using “bright” to refer to quantum yields, since brightness is actually a function of quantum yield and the extinction coefficent for absorption. If a fluorophore has a 99% quantum yield but very low light absorption, would you still think of it as bright?

    Comment by Andrew — October 29, 2009 #

  4. “bright” is not a scientific term. but the word does imply that both the extinction coeff and the QY are high. if i had to define it, fluorescence “brightness”—or really the fluorescence irradiance—is the rate of photon emission, which in turn depends on the excitation irradiance, the extinction coeff, the quantum yield, the fluorescence lifetime, the rate of intersystem crossing, the triplet lifetime, ….

    bottom line: i can see fluorescence from single molecules of these dyes inside living cells. that’s “bright.”

    Comment by sam — October 29, 2009 #

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