how Cy5 switches
December 7, 2009 at 9:10 am | sam | literature, single moleculesXiaowei Zhuang and Roger Tsien have published a JACS paper on how Cy5 photoswitches: “Photoswitching Mechanism of Cyanine Dyes.”
Nothing surprising here: this is the mechanism that Tsien proposed informally a couple years ago. But now they have published evidence that the thiol (e.g. BME, a component of the oxygen-scavenging system) attacks a polymethene bond.
I think this paper is a nice characterization of the photochemistry involved in Cy5 photoswitching.
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