3-d surface

April 11, 2006 at 4:31 pm | | cool results, everyday science, single molecules, software

A fellow labmember showed me how to use MatLab to make a 3-dimensional surface from an image of single fluorescent molecules, where z is the intensity. Here’s a taste. I converted this:

sm
into this:
smsurf

The funny thing is that the pretty image is only that: it actually contains less information than the 2-d grayscale image, becuase it needed smoothing and convolving with Gaussians to make it look so nice. But that kinda shit brings in the big $$.

3 Comments »

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  1. The 2-d image contains information? Seriously? But the 3-d image is much, much prettier!

    Seriously, let’s be frank. When I do spectroscopy, my S/N ratio is just so much better than in single-molecule spectroscopy!

    Comment by charles — April 12, 2006 #

  2. I so science for the pretty picture, not for SNRs!

    Comment by sam — April 12, 2006 #

  3. use WCIF image J.
    http://www.uhnresearch.ca/facilities/wcif/fdownload.html

    Comment by singlebiophysicist — November 11, 2006 #

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