Great paper from my previous lab. And with a ridiculously hilarious acronym (a play on Hochstrasser’s PAINT): superresolution by power-dependent active intermittency and points accumulation for imaging in nanoscale topography (SPRAIPAINT). This acronym fails almost all my “GINAP” rules for initialisms, but I still love it because it joins the plethora of acronyms in the super-resolution microscopy field: PALM, FPALM, STORM, dSTORM, STED, GSDIM, PAINT, RESOLFT, SMACM, FIONA, SHREC, SHRIMP, SIM, SOFI, NALMS, … and I’m sure I’m forgetting some. This acronym shitstorm certainly deserves more. In all honesty, I think we should drop all the acronyms and just call it “pointillist super-resolution microscopy.”
The images in this paper are just beautiful! The bacteria they image are very small, basically at the diffraction limit of a conventional microscope. But they are able to image three-dimensional helices of protein filaments inside these tiny guys!
The movies are awesome:
Cool. Keep up the good work, Moerner lab.
Top three safety rules, especially for new students:
- If you’re unsure about any safety issue, ask someone!
- Wear safety glasses when freezing things.
- Wear a face shield when piranha etching.
An addendum rule is to not work sloppily in general. Or when you’re very tired.
Of course, there are many other important rules. But these are my favs.